Keywords: Step3.gif en 3 Transfer 20 µl from well 10 to well 9 mix properly and transfer 20 µl to well 8 etc until coming to well 3 which is the last well for the dilution series After mixing discard 20 µl from well 3 This generates a dilution series 1/1 1/2 ¦ 1/128 Wells 1 and 2 contain only the solvent Gentaur Gentaur Biochemistry diagrams |