Keywords: Gel shift assay.png Lane 1 is a negative control and contains only DNA Lane 2 contains protein as well as a DNA fragment that is based on its sequence does not interact Lane 3 contains protein and a DNA fragment that does react; the resulting complex is larger heavier and slower-moving The pattern shown in lane 3 is the one that would result if all the DNA were bound and no dissociation of complex occurred during electrophoresis When these conditions are not met a second band might be seen in lane 3 reflecting the presence of free DNA or the dissociation of the DNA-protein complex Created by user Ganaaaa 9-30-2005 I the creator of this image hereby release it into the public domain This applies worldwide 2005-09-30 Transferred from http //en wikipedia org en wikipedia by Ronhjones Ganaaaa at en wikipedia other versions Ganaaaa Original upload log page en wikipedia Gel_shift_assay png Upload date User Bytes Dimensions Comment 2005-09-30 23 04 UTC Ganaaaa 9174 bytes 672×468 <small><nowiki>Created by user Ganaaaa 9-30-2005 I the creator of this image hereby release it into the public domain This applies worldwide </nowiki></small> Scientific techniques Electrophoresis Evolution Biochemistry methods Proteins Proteomics |