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| Keywords: Figure-1.JPG en Figure 1 Overview of the bacterial one-hybrid system A library of randomized nucleotides representing potential transcription factor binding sites 10-20 base pairs the prey is cloned upstream of HIS3 and URA3 positive and negative selectable markers respectively If the transcription factor binds to the randomized region it will recruit RNA polymerase via direct fusion to the α- or ω-subunit and thus activate transcription of the downstream reporter genes The host E coli strain being used must lack the bacterial homologues of these biosynthetic genes HIS3 and URA3 own Graeme Bell Noushin Moshgabadi 2009-02-21 Bacterial one-hybrid system Diagrams in English | ||||
